Midterm 1 Answers - 2006 - Ion exchange chromatography. For...

Info iconThis preview shows pages 1–16. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
The C-terminal end will carry a negative charge, and the N-terminal end will carry a positive charge due to the helical dipole. Therefore, positively charged residue B (lysine) will interact with the C-terminus, and negatively charged residue A (aspartate) will interact with the N-terminus.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 10
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 12
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 14
Background image of page 15

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 16
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Ion exchange chromatography. For example, if a positively-charged column is used at pH 7, protein B will be negatively charged while protein C will be (close to) neutral. Therefore, protein B will stick to the positively charged column while protein C will elute from the column rst....
View Full Document

This note was uploaded on 01/27/2011 for the course MCB 100A taught by Professor Kuryian during the Spring '09 term at University of California, Berkeley.

Page1 / 16

Midterm 1 Answers - 2006 - Ion exchange chromatography. For...

This preview shows document pages 1 - 16. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online