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Homework+2+Revised - polysaccharide of D-glucosamine...

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Homework 2: Due Friday, Sept. 17 th Chemical Engineering 170A: Biochemical Engineering Question 1. Kinetic Parameters for Trypsin and a Catalytic Antibody. Blanch and Clark Reader Problem 3 Question 2. Transition State Stabilization Consider the following hypothetical reaction, the creation of a heart shape. Substrate Product Sketch and label the free energy diagram for this reaction without an enzyme present. Then sketch and label the free energy diagrams for the reaction, assuming that it is individually catalyzed by the enzymes below (a and b). Compare them on the diagram you draw and explain why you drew the diagrams differently or the same. a) b) Question 3. Inhibition kinetics. Several compounds are tested in a study on a lipase from the fungus Candida rugosa to find an inhibitor that suppresses its lipolytic activity. One compound that shows promise is chitosan, a
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Unformatted text preview: polysaccharide of D-glucosamine residues. Another compound, N-succinylchitosan, also shows promise. From the data below, answer the following questions: S Velocity (mmol min-1 ) (mM) No Inhbitior Chitosan N-succinylchitosan 0.27 0.063 0.046 0.041 0.40 0.080 0.061 0.055 0.83 0.111 0.097 0.089 1.0 0.125 0.106 0.099 1.3 0.143 0.120 0.112 2.0 0.167 0.140 0.134 a. What are the values of v max and K M without an inhibitor? With chitosan? With N-succinylchitosan? [I] = 0.3 mM b. What is the binding constant of each inhibitor? c. If S = 0.1 mM and [I] chitosan = 1 mM, what fraction of the enzyme molecules have a bound substrate? A bound inhibitor? Question 4. Rate Equation for Combined Inhibition. Blanch and Clark Reader Problem 7...
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