SI BIO100 MID 2 Review FINAL

SI BIO100 MID 2 Review FINAL - SI Session BCH100 SI leader:...

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SI Session BCH100 SI leader: Sumeet Spring 09 WARNING: DO NOT USE THIS AS YOUR ONLY SOURCE FOR STUDYING, YOU MUST GO THROUGH DR. LARSENS LECTURE NOTES AND UNDERSTAND ALL THE CONCEPTS AND BE ABLE TO APPLY THEM. YOU WILL NOT DO WELL BY JUST STUDYING THIS GUIDE!!! THE ULTIMATELY SUPER AWESOME MIDTERM# 2 REVIEW 1. What is metabolism? - Biological process that consists of breaking down or building up molecules - Catabolism(oxidative, exergonic) – break down of macromolecules - Anabolism(reductive, endergonic) – build up of macromolecules 2. What role does NAD+/NADH play in the biological process you have studied? Used in catabolic type of reactions, NAD+ is an oxidizing agent that is oxidizes other compounds in the glycolysis and TCA and becomes reduced to NADH. FAD is also used as an oxidizing agent that oxidizes other compounds and is seen in TCA. Note( You must be able to recognize FAD/FADH2, and NAD+/NADH 3. How do you drive an endergonic reaction in order for it to proceed? You may drive an endergonic reaction by coupling it with enough ATP. Taking an ATP molecule and Hydrolizing it will release energy as ATP is broken into ADP + Pi. The overall Free energy released is - 31 kj/mol. Ex. E + S ES ∆G = 29 kj/mol ATP + H2O ADP + Pi ∆G = -31 kj/mol The process can now be driven because the overall ∆G is negative and now spontaneous. 4. Things to know about the biological process that you have studied. Glycolysis(catabolic):Breakdown of a glucose molecule. Net result = 2ATP, 2 NADH, & 2 Pyruvate. Contains 10 steps and occurs in the cytosol of the cell. Anaerobic process.
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                                                        Regulatory steps: Steps 1,3, 10 Step 1 is regulated by negative feedback due to build up of G-6-P which inhibits hexokinase via negative feed back Step 3 is a committed step that is regulated by allosteric effectors such as ATP and F-2,6-BP                                             Step 10 is also allostericly regulated by ATP as well
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Gluconeogenesis is an anabolic process involved TCA occurs in the matrix of the In building up a glucose molecule in order to mitochondria. Pyruvate is introduced store up energy in the form of glucose which can into the cycle via conversion to later be used to form a large macromolecule used to Acetyl CoA( You should know how this store glucose for long term purposes. This occurs). Eight Steps in the cycle, I will macromolecule is known as glycogen. Reverse process indicate step 1 as starting from in which Step 10 of glycolysis has now become step 1 Oxaloacetate, but really there is no of gluconeogenesis. Contains 11 Steps. 4 different starting point for TCA after AcetylCoA steps than glycolysis. Has been introduced. TCA produces 3 NADH, 1 FADH2, 1GTP per AcetylCoA that enters. 5.
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SI BIO100 MID 2 Review FINAL - SI Session BCH100 SI leader:...

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