4.1.3.A.SRS StudentResponse.pdf - Activity 4.1.X Student Response Sheet Answer the questions below as you work your way through each step of the GFP

4.1.3.A.SRS StudentResponse.pdf - Activity 4.1.X Student...

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Activity 4.1.X Student Response Sheet Answer the questions below as you work your way through each step of the GFP Purification laboratory. Be sure to think about how all of the steps function together to isolate the protein of interest. NOTE: The experiment begins with Lesson 2. Lesson 1 covers the background information for the lab experiment and not the actual hands-on part of the work. Lesson 2 – Inoculation Goal: ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ 1. What would happen if you took one of the green colonies from your LB/amp/ara plate and streaked it on a LB/amp plate? Conversely, what would happen if you took a white colony from the LB/amp plate and streaked it on an LB/amp/ara plate? Explain your answer. 2. Explain how an incubator helps with this cloning experiment. Lesson 3 – Purification Phase 1: Bacterial Concentration Goal: ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ © 2010 Project Lead The Way, Inc . Medical Interventions Activity 4.1.3 Student Response Sheet – Page 1
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1. Explain the role of lysozyme and the freezing of the cells play in the isolation the GFP from the other bacterial proteins.
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