3500Ch20 - 11 Great movie – but missing something… • http/vcell.ndsu.nodak.edu/animations/e 22 The Chemiosmotic Theory • Proposed by Peter

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Unformatted text preview: 11 Great movie – but missing something…. • http://vcell.ndsu.nodak.edu/animations/e 22 The Chemiosmotic Theory • Proposed by Peter Mitchell in the 1960’s • Nobel Prize 1978 • Chemiosmotic theory : A proton concentration gradient serves as the energy reservoir for driving ATP formation 44 Mitchell’s postulates for chemiosmotic theory 1. Intact inner mitochondrial membrane is required to maintain a proton gradient 2. Electron transport through the ETC generates a proton gradient (pumps H+ from the matrix to the intermembrane space) 3. The membrane-spanning enzyme, ATP synthase, catalyzes the phosphorylation of ADP in a reaction driven by movement of H+ across the inner membrane into the matrix NOTE: Both the pH and charge difference are important. 55 The Protonmotive Force • Protonmotive force ( ∆ p ) is the energy of the proton concentration gradient (analogous to the electromotive force used in electrochemistry) • H+ flow forms a circuit (into the intermembrane space by electron transport, flow back into the matrix via ATP synthase) • The total energy of the membrane state has both chemical and electrical energy 66 Coupled nature of respiration in mitochondria Coupled : Either both reactions occur, or neither occurs Ø Oxidation of substrates is coupled to the phosphorylation of ADP Ø Respiration (consumption of oxygen) proceeds only when ADP is present (ie they are coupled) Ø O2 consumed depends on ADP added Put to the test! • Bacteriorhodopsin is activated to pump protons in response to light. • When the synthetic vesicles to the right were made they were able to make ATP when exposed to light. 88 Complex V: ATP Synthase • A.k.a. F0 F1 ATPase • Uses the proton gradient energy for the synthesis of ATP • Composed of a “ ball and stick ” structure...
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This note was uploaded on 02/03/2011 for the course CHEM 3500 taught by Professor Staff during the Spring '08 term at Kennesaw.

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3500Ch20 - 11 Great movie – but missing something… • http/vcell.ndsu.nodak.edu/animations/e 22 The Chemiosmotic Theory • Proposed by Peter

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