3000test - Brittney Brown 2d.Simultaneous determination of...

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Brittney Brown 2d. Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed micelle capillary electrophoresis By: Flor, Sabrina; Lucangioli, Silvia; Contin, Mario; Tripodi, Valeria A new CE system based on the use of polymeric-mixed micelles (cholic acid, SDS and the poloxamine Tetronic 1107) was developed for the simultaneous detn. of nine steroids in human urine. This method allows the baseline sepn. and quantitation of cortisol, androstenedione, estriol, dehydroepiandrosterone sulfate, testosterone, dehydroepiandrosterone, estrone, progesterone and estradiol in less than 25 min showing to be sensitive enough to detect low concns. of these steroids in urine samples (5-45 ng/mL). The optimized electrophoretic conditions were performed using a 50 cm × 75 μm capillary, 18 kV, 25°C, with 44 mM cholic acid, 10 mM SDS, 0.05% w/v tetronic 1107, 2.5% vol./vol. methanol, 2.5% vol./vol. THF in 5 mM borate - 5 mM phosphate buffer (pH = 8.0) as a background electrolyte and a dual 210/254 UV-detection. The method can simultaneously det. 0.1-120 μg/mL, which corresponds to 5-6000 ng/mL of steroids in 2 mL urine. The recoveries ranged between 82.4 and 101.5%. Due to its simplicity, speed, accuracy and reliability, the proposed method could be a potential alternative to the traditional methodologies used with clin. purposes. 3. Mechanism-based selection of compounds for the development of innovative in vitro approaches to hepatotoxicity studies in the LIINTOP project By: Gomez-Lechon, Maria Jose; Tolosa, Laia; Castell, Jose V.; Donato, Maria Teresa
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This note was uploaded on 02/03/2011 for the course CHEM 3000 taught by Professor Staff during the Fall '08 term at Kennesaw.

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3000test - Brittney Brown 2d.Simultaneous determination of...

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