Simultaneous determination of nine endogenous steroids in human urine by polymeric-mixed
micelle capillary electrophoresis
By: Flor, Sabrina; Lucangioli, Silvia; Contin, Mario; Tripodi, Valeria
A new CE system based on the use of polymeric-mixed micelles (cholic acid, SDS and the poloxamine
Tetronic 1107) was developed for the simultaneous detn. of nine steroids in human urine. This method
allows the baseline sepn. and quantitation of cortisol, androstenedione, estriol, dehydroepiandrosterone
sulfate, testosterone, dehydroepiandrosterone, estrone, progesterone and estradiol in less than 25 min
showing to be sensitive enough to detect low concns. of these steroids in urine samples (5-45 ng/mL).
The optimized electrophoretic conditions were performed using a 50 cm × 75 μm capillary, 18 kV, 25°C,
with 44 mM cholic acid, 10 mM SDS, 0.05% w/v tetronic 1107, 2.5% vol./vol. methanol, 2.5% vol./vol.
THF in 5 mM borate - 5 mM phosphate buffer (pH = 8.0) as a background electrolyte and a dual 210/254
UV-detection. The method can simultaneously det. 0.1-120 μg/mL, which corresponds to 5-6000 ng/mL
of steroids in 2 mL urine. The recoveries ranged between 82.4 and 101.5%. Due to its simplicity, speed,
accuracy and reliability, the proposed method could be a potential alternative to the traditional
methodologies used with clin. purposes.
Mechanism-based selection of compounds for the development of innovative in vitro approaches to
hepatotoxicity studies in the LIINTOP project
By: Gomez-Lechon, Maria Jose; Tolosa, Laia; Castell, Jose V.; Donato, Maria Teresa