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Spectrophotometric Analysis of a Mixture: Caffeine and Benzoic Acid in   Cola CHEM 2800.01 Lab Experiment Date – October 23, 2008 Report Date – October 30, 2008
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Report Due – October 30, 2008 Introduction: Using Beer’s Law (equation 1), there is a direct connection between absorptivity and  concentration.  The problem with Beer’s law is that it is additive (equation 2) and two  substances can affect the same wavelength at the same time.  Because of this additive  effect it is impossible to directly find the correlation between the absorptivity and  concentration of two substances that overlapping on the same frequency. Equation 1.  A bC Equation 2.  A 1 bC 1 + ε 2 bC 2 + .. + ε b bC n This lab is interested in the concentration of Caffeine and Benzoic Acid in Mountain  Dew.  Caffeine is an additive known to depress the effects of exhaustion.  Benzoic acid  is used as a food preservative in the beverage.  The issue is that the absorptivity curves  of these two components over lap each other (see chart 1). In order to determine the concentration of Caffeine and Benzoic Acid a series of steps  must be preformed.  Using pure caffeine and benzoic acid, calibration curves were  created for these substances at two wavelengths.  These wavelengths were chosen  because of the high response of these two substances.  Then the absorptivity of the  CHEM 2800.01L Page 2 of 7
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Report Due – October 30, 2008 Mountain Dew in a diluted form was obtained.  Using the calibration curves, the  absorptivity of the Mountain Dew at the chosen wavelengths and some linear regression 
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This note was uploaded on 02/03/2011 for the course CHEM 2800 taught by Professor Staff during the Fall '08 term at Kennesaw.

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