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Unformatted text preview: 1 Spectrophotometric Analysis of a Mixture of Caffeine and Benzoic Acid in Cola CHEM 2800.01 Lab Experiment Date – November 1, 2010 Report Date – November 8, 2010 Introduction: The purpose of this experiment is to measure the content of Benzoic acid and Caffeine in soft drinks by using ultraviolent absorbance.  It is also to determine the amount of each of these substances in an unknown sample though beers law and our known samples. The absorbance of a solution that contains more than one chromophore or substance the total absorbance will be equal to each component the substance contributes at any given wavelength. So the concentration of each chromophore can be determined by measuring the total absorbance at a particular wavelength and solving the matrix equations (2) and (3) below when the optical absorptive of the individual components are known. Since only caffeine and benzoic acid are being analyzed n=2 so only two equations are needed. Equation (1.) n n t C b C b C b A ε ε ε + + + = ... 2 2 1 1 Equation (2.) n C C C A n , 1 2 , 1 1 , 1 1 ......
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This note was uploaded on 02/03/2011 for the course CHEM 2800 taught by Professor Staff during the Fall '08 term at Kennesaw.
- Fall '08