2800cola - SPECTROPHOTOMETRIC ANALYSIS OF CAFFEINE AND...

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SPECTROPHOTOMETRIC ANALYSIS OF CAFFEINE AND BENZOIC ACID IN COLA BRITTNEY BROWN, KENNESAW STATE UNIVERSITY, KENNESAW, GEORGIA. PARTNER: ERICA FRANCIS CHEM 2800L November 10, 2010
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Brown 11/10/2010 INTRODUCTION: The purpose of this experiment was to quantitatively determine the amount two major species, Caffeine and Benzoic Acid, in a soft drink using ultraviolent absorbance. Caffeine is an addictive used as a stimulant and benzoate is used as a food preservative in the beverage. In order to determine the concentration of caffeine and benzoic acid a series of dilution steps were performed. Calibration curves were created for pure samples of caffeine and benzoic acid. Based on Beer’s law, the absorbance of the solution that contains more than one chromophore will equal the sum of the absorbances of each component at a given wavelength (λ m ) using Equation (1): (1) By knowing the optical path length and optical absorptivities of the individual components, the concentration of each chromophone can be determined by measuring the total absorbance at a given wacelength and solving for the matrix using Equation (2) and (3): (2) (3) Using the calibration curves, the absorptivity of the caffeine and benzoic acid in the soft
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2800cola - SPECTROPHOTOMETRIC ANALYSIS OF CAFFEINE AND...

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