10-13 Genomes and Their Evolution

10-13 Genomes and Their Evolution - 10/11/2010 1 Genomes...

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Unformatted text preview: 10/11/2010 1 Genomes and their evolution Chapter 21 pp 426-449 Sequencing Determine the complete nucleotide sequence of every chromosome: 1, 2, 3, 4, 5, 6,. Tallest to shortest ACTGAATGGGATCTCA Early genomes sequenced E. coli Saccharomyces cervisiae Caenorhabditis elegans Drosophila melanogaster Mus musculus Macaca mulatta Homo sapiens human genome project 1990 to 2003 & 2006 Genomics & bioinformatics Genomics : study of whole sets of genes & their interactions Bioinformatics application of computational methods for storage and analysis of enormous data sets Data banks (databases) www.ncbi.nlm.nih.gov What is the purpose of all of this? 10/11/2010 2 Genome sequencing: 3 Stage sequential approach Karyotyping FISH : fluorescence in situ hybridization w/ labeled probes Produced a cytogenetic map 1) Linkage map : Order and distance between markers (RFLP s & STR s) in DNA 2) Physical map ordering of overlapping fragments: larger & smaller 3) DNA sequencing of nucleotide sequence of small fragments Fig. 21-2-4 Cytogenetic map Genes located by FISH Chromosome bands Linkage mapping 1 2 3 Genetic markers Physical mapping Overlapping fragments DNA sequencing Whole genome shotgun approach Craig Venter & Celera Genomics Based on first complete genome sequence of an organism in 1995 Haemophilus influenzae Race with NIH to completely sequence the Human genome-some errors Reminiscent of the CODE WARS 10/11/2010 2 Genome sequencing: 3 Stage sequential approach Karyotyping FISH : fluorescence in situ hybridization w/ labeled probes Produced a cytogenetic map 1) Linkage map : Order and distance between markers (RFLP s & STR s) in DNA 2) Physical map ordering of overlapping fragments: larger & smaller 3) DNA sequencing of nucleotide sequence of small fragments Fig. 21-2-4 Cytogenetic map Genes located by FISH Chromosome bands Linkage mapping 1 2 3 Genetic markers Physical mapping Overlapping fragments DNA sequencing Whole genome shotgun approach Craig Venter & Celera Genomics...
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10-13 Genomes and Their Evolution - 10/11/2010 1 Genomes...

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