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9-30-10 Lecture 3

9-30-10 Lecture 3 - Stained with a uorescent dye Figure...

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Figure 9-13 Molecular Biology of the Cell (© Garland Science 2008) Stained with a fuorescent dye
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Different ways to label cells so that they Fuoresce • Stain cells with antibodies that have been labeled with Fuorescent dyes • Stain cells with a Fuorescent dye that stains a particular class of molecules • Make a fusion protein with a Fuorescent protein
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Figure 9-18 Molecular Biology of the Cell (© Garland Science 2008)
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Mongoose Skin Fibroblast Cells 3 different ±uorescent dyes staining 3 different structures
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Figure 9-15 Molecular Biology of the Cell (© Garland Science 2008) Micrograph of cell in mitosis 3 ±uorescent probes Spindle microtubules - green Centromeres -red DNA -- blue
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Found in Jellyfish -- Aequorea victoria Green Fluorescent Protein The bundled actin structures poking out from the leading edge of the neuron are known as Flopodia,
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Figure 9-24 Molecular Biology of the Cell (© Garland Science 2008)
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Figure 9-14 Molecular Biology of the Cell (© Garland Science 2008) Fluorescent dyes absorb light of one wavelength and emit light of a longer (lower energy) wavelength
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Figure Q9-3 Molecular Biology of the Cell (© Garland Science 2008) Rainbow colors produced by modifed GFPs
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Alba -- Fluorescent bunny
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Figure 9-20 Molecular Biology of the Cell (© Garland Science 2008)
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9-30-10 Lecture 3 - Stained with a uorescent dye Figure...

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