10-19-10 Lecture 7

10-19-10 Lecture 7 - Office Hours Tues 11.40 AM – 12.30...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Office Hours Tues 11.40 AM – 12.30 PM Thur 3.00 – 3.50 PM LSA3114 LSA3114 How a soluble protein is translocated across the ER membrane Start transfer signal Figure 12-45 Molecular Biology of the Cell (© Garland Science 2008) How a single-pass transmembrane protein with a cleaved ER signal is integrated into the ER membrane. Figure 12-46 Molecular Biology of the Cell (© Garland Science 2008) Integration of a single pass transmembrane protein with an internal signal sequence into the ER membrane +vely charged aa preceding start-transfer seq +vely charged aa following start-transfer seq Figure 12-47 Molecular Biology of the Cell (© Garland Science 2008) Integration of double-pass transmembrane protein with an internal signal sequence into the ER membrane Figure 12-48 Molecular Biology of the Cell (© Garland Science 2008) Insertion of the multipass membrane protein into the ER membrane Figure 12-49 Molecular Biology of the Cell (© Garland Science 2008) What happens to proteins in the ER? Soluble protein and membrane spanning proteins are modified by enzymes in the lumen: •  Folding and formation of disulfide bonds •  Addition of GPI anchor •  Glycosylation •  Quality control ER resident protein – PDI (protein disulfide isomerase) Disulfide bonds CXXC Figure 3-28 Molecular Biology of the Cell (© Garland Science 2008) N-linked glycosylation Two types of sugar addition: N-linked O-linked Potential site of glycosylation: NXS/T N-linked precursor oligosaccharide Figure 12-50 Molecular Biology of the Cell (© Garland Science 2008) Protein glycosylation in the rough ER Figure 12-51 Molecular Biology of the Cell (© Garland Science 2008) Role of N-linked glycosylation in ER protein folding Calreticulin Figure 12-53 Molecular Biology of the Cell (© Garland Science 2008) Export and degradation of misfolded ER proteins Retrotranslocation Mannosidase Figure 12-54 Molecular Biology of the Cell (© Garland Science 2008) Attachment of GPI anchor to a protein in the ER Figure 12-56 Molecular Biology of the Cell (© Garland Science 2008) Vesicular Trafficking Figure 13-1 Molecular Biology of the Cell (© Garland Science 2008) Secretory and endocytic pathways Figure 13-3b Molecular Biology of the Cell (© Garland Science 2008) Vesicular transport Figure 13-2 Molecular Biology of the Cell (© Garland Science 2008) Vesicle types Use of different coats in vesicular traffic Figure 13-5 Molecular Biology of the Cell (© Garland Science 2008) Electron micrograph of different vesicles Figure 13-4 Molecular Biology of the Cell (© Garland Science 2008) Structure of clathrin coat Triskelion Figure 13-7a, b Molecular Biology of the Cell (© Garland Science 2008) Structure of clathrin coat Figure 13-6 Molecular Biology of the Cell (© Garland Science 2008) Structure of clathrin coat Figure 13-7c, d Molecular Biology of the Cell (© Garland Science 2008) Assembly and disassembly of clathrin coat Figure 13-8 Molecular Biology of the Cell (© Garland Science 2008) Role of dynamin in pinching off clathrin-coated vesicles Figure 13-12a Molecular Biology of the Cell (© Garland Science 2008) Movie clathrin coated vesicles ...
View Full Document

This note was uploaded on 01/31/2011 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.

Ask a homework question - tutors are online