CBNS120 discussion 2

CBNS120 discussion 2 - CBNS 120 Discussion Week 2 Alison...

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Unformatted text preview: CBNS 120 Discussion Week 2 Alison Xiaoqiao Xie xiaoqiao.xie@email.ucr.edu Office hours: Friday 2-4 PM, BSB 1105 H W 2: Descr ibe 3 mechanisms cells use to pr oduce diver sity of ion channel str uctur e and function.-- How do cells produce ion channel? (hint: ion channels are proteins) iver sity of genes expressing different subtype of channels (nucleotide sequence differ lternative splicing of exons from the same gene NA editing 1. I solation of proteins: enriched area, detergents and even denaturing agents for extraction, high affinity toxins used for isloation 2. Molecular cloning to determine amino acid sequence: Construction of cDNA Library 3. Site directed mutagenesis and expression in oocytes for determining the conformation and study the properties. 4. New tech: electron microscopy, X-ray diffraction (Fig 3.1) Examples: nAchR and K+ channel H W 3: What exper imental appr oaches have been used to obtain the amino acid sequences of ion channels, for example the nicotinic choliner gic r eceptor and the shaker K channel? H W 4: Dr aw a diagr am of the muscle nicotinic acetylcholine r eceptor (nAChR) showing its subunit composition. To which subunit does acetylcholine (ACh) bind? H ow many ACh binding sites ar e pr esent on the nAChR? H W 5: H ow does the subunit composition of the neur onal nAChR differ fr om that of the muscle nAChR?...
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CBNS120 discussion 2 - CBNS 120 Discussion Week 2 Alison...

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