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CBNS120 discussion 2

CBNS120 discussion 2 - C BN S 120 D iscussion W eek 2...

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CBNS 120 Discussion Week 2 Alison Xiaoqiao Xie [email protected] Office hours: Friday 2-4 PM, BSB 1105
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HW 2: Describe 3 mechanisms cells use to produce diversity of ion channel structure and function. -- How do cells produce ion channel? (hint: ion channels are proteins) iversity of genes expressing different subtype of channels (nucleotide sequence differ lternative splicing of exons from the same gene NA editing 1. Isolation of proteins: enriched area, detergents and even denaturing agents for extraction, high affinity toxins used for isloation 2. Molecular cloning to determine amino acid sequence: Construction of cDNA Library 3. Site directed mutagenesis and expression in oocytes for determining the conformation and study the properties. 4. New tech: electron microscopy, X-ray diffraction (Fig 3.1) Examples: nAchR and K+ channel HW 3: What experimental approaches have been used to obtain the amino acid sequences of ion channels, for example the nicotinic cholinergic receptor and the shaker K channel?
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HW 4: Draw a diagram of the muscle nicotinic acetylcholine receptor (nAChR) showing its subunit composition. To which subunit does acetylcholine (ACh) bind? How many ACh binding sites are present on the nAChR? HW 5: How does the subunit composition of the neuronal nAChR differ from that of the muscle nAChR?
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