lecture 5 - Methods to Measure Cell Growth Direct Methods...

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Methods to Measure Cell Growth • Direct Methods – Total Cell Counts (live + dead) – Viable Cell Counts (live only) • Indirect Methods – Turbidity (live + dead)
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Counting Bacteria • Petroff-Hauser chamber – Hemacytometer • Need a microscope – Counts cells directly – Gives the accurate number – Can’t tell whether cells are alive or dead • Use staining to distinguish living cells
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Viable Counts – Counts only those cells able to reproduce – Make serial dilutions from liquid culture – Spread each dilution on solid medium • Count the number of colonies • Each colony is originated from a single live cell in the original culture • Can only accurately count 30- 300 colonies/plate
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Too few to count Unknown concentration (cfu/mL) # of colonies X dilution factor Volume spread on the plate 311 x 10 3 27 x 10 4 0.1 mL 0.1 mL
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Counting Bacteria • Spectrophotometer – Measures optical density – turbidity • “Shadow” of bacteria –G ives rapid
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This note was uploaded on 01/31/2011 for the course BIO 121 taught by Professor Ma during the Winter '10 term at UC Riverside.

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lecture 5 - Methods to Measure Cell Growth Direct Methods...

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