The purpose of the “Bacteriophage multiplication; one step growth” is to be able to determine the different phases that take place during the one step growth and to know what takes place during each phase. In addition, be able to calculate the average burst size. Latent period is the initial phase in the one step growth experiment in which no phages are released Eclipse period is the initial part of the latent period I which infected host bacteria do not contain any complete virions. Intracellular accumulation period is the phase the nucleic acid and structural proteins that have been made are assembled and infectious phage particles accumulate within the cell. Rise/burst period is the period during the one step growth experiment when host cells lyse and release phage particles. The plague count dropped at the very beginning of the infectious cycle when the infected culture is treated with chloroform because chloroform disrupts membrane, releasing cell contents including the assembled phage. Since, bacteriophage lack membrane, therefore they are not killed by the
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