Culture Media, Enzymes, Fermentation

Culture Media, Enzymes, Fermentation - CULTURE MEDIA The...

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CULTURE MEDIA The purpose of the “Culture Media” lab is to become familiar with the various types of media, which include general purpose, synthetic and selective media. In addition, to be able to determine the types of microbes that are capable of developing on different media. The ability of microbes to develop depends on the ingredients that are present in media, which may or may not be suitable for microbe growth. The SF medium is a type of selective medium used to specifically select for Enterococcus (Streptococcus) faecalis . The two organisms used to test to see whether or not growth would occur on the SF broth medium were the Staphylococcus aures and Enterococcus faecalis . Streptococci are immune to chemicals that inhibit electron transfer along the respiratory chain, one of which is the sodium azide, which is the ingredient present in the broth which aids in the “selectiveness” for Enterococcus faecalis . The sodium azide prevents the growth of microorganisms that have a respiratory chain. Brom cresol purple is an ingredient present in the selective medium because it is an acid indicator that changes color when fermentation of glucose has occurred. The color change from purple to yellow indicates growth of Enterococcus faecalis occurred and no growth occurred for Staphylococcus aures because there was no color change. MacConkey Agar is a type of differential media used to differentiate certain bacteria from others. The organisms used to test to see whether or not growth would occur on the MacConkey Agar were Enterococcus faecalis, Salmonella enteric, Pseudomonas aeruginosa, Esherichia coli and Bacillus subtilis. All the organisms tested, exhibited growth on the MacConkey Agar except for Enterococcus faecalis because the section where it was inoculated did not show any sign of growth as opposed to sections with clearing zones and color changes which indicated growth. Enterococcus faecalis is a gram positive organism; bile salts and crystal violet were ingredients in the MacConkey agar, both of which inhibit the growth of gram positive organisms. Therefore,
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This note was uploaded on 01/31/2011 for the course MCB 2000 taught by Professor Bruno, m during the Spring '08 term at UConn.

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Culture Media, Enzymes, Fermentation - CULTURE MEDIA The...

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