Motility The purpose of the “motility” lab is to verify whether or not the organisms, Bacillis subtilis, Pseudomonas aeruginosa and Staphylococcus aures display motility under the wet mount, hanging drop slide and motility agar methods. The wet mount was conducted by placing a few loopfuls of the culture on a slide with a cover slip and observed using a microscope. All the Bacillis subtilis and Pseudomonas aeruginosa were organisms that displayed motility in the wet mount. The organism Staphylococcus aures did not possess any motility. In order to view the flagella, it was necessary to decrease the bright illumination by increasing the contrast because the flagella are very thin and translucent structures. The hanging drop preparation is a method conducted to view motility without the oxygen depletion evaporation of the wet mounts. The organisms that exhibited motility were Bacillis subtilis and Pseudomonas aeruginosa . The motility agar is conducted to verify the motile organisms from the non motile organisms through observation. A sample of each
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