zool 4241 -proposed Renilla Protocol[2]

zool 4241 -proposed Renilla Protocol[2] - Renilla 5 times...

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September 30, 2009 Zoology 4241 Renilla Protocol N=24 Night before the Renilla go into a smaller tank that goes into the refrigerator at 8 0 Celsius. Before beginning experiment room will be blacked out for approximately 15 minutes to get all participants acclimated to the dark –TURN OFF CELL PHONES OR ANY LIGHT PRODUCING DEVICES BEFORE ENTERING LAB—ONLY RED LIGHTS ALLOWED FOR USE IN SEEING TO RECORD RESULTS Independent Variables a. (M) - Air/H2O (using a 6 cc syringe) i. Randomly assign order of IV stimulation. Make sure syringe is utilized in the center of Renilla without touching. ii. 3 cc Air/H2O at 3 seconds iii. 6 cc Air/H2O at 3 seconds b. (M) - Rod i. Touch the
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Unformatted text preview: Renilla 5 times in the center ii. Touch the Renilla 5 times in the center and moving outward in a stroking manner c. (E) - Electric volts i. 2 leads positive and negative ii. Electrodes placed on each side of the Renilla , hold on location. After luminescence, wait 30 seconds to increase voltage, and then repeat. iii. One shock should be held for 10 milliseconds, then double the voltage. (0, 2, 4, 6, 8, 10) • Dependent Variables o Time of B.I. o Pattern (only point where stimulated or wave through entire colony) September 30, 2009 Zoology 4241 N – population=24 B.I. - bioluminescence (M) - mechanical (E) - electrical...
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This note was uploaded on 02/01/2011 for the course ZOOL 4241 taught by Professor Lab during the Spring '08 term at Texas Woman's University.

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zool 4241 -proposed Renilla Protocol[2] - Renilla 5 times...

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