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Unformatted text preview: describe the role of this chemistry in the folding of proteins. Answer question 4. It is worth 6 marks. 4. Explain how Immobilized Metal Affinity Chelate Chromatography can be used to purify proteins. Answer question 5. It is worth 6 marks. 5. Give a definition of the “hydrodynamic particle”. Briefly, but clearly, explain the difference between sedimentation velocity and sedimentation equilibrium analysis of proteins emphasizing the information obtained from each method. Answer question 6. It is worth 6 marks. 6. From the data given in the mass spetrum below calculate the mass of the protein. For full marks show your calculations. What method was used to introduce the protein into the mass spetrometer? Answer question 7. It is worth 4 marks. 7. With the use of the following equation explain how time-of-flight mass spectrometry measures the masses of proteins: m z = 2 Vt 2 L 2...
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