Lecture11_1 - 2/3/11 Main Points Brief review of...

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Unformatted text preview: 2/3/11 Main Points Brief review of translation Bacterial gene regulation- operons The many levels of eukaryotic gene 2/3/11 Fig. 17-13 Polypeptid e Riboso me Ami no acid s tRNA with amino acid attached tRN A Anticod on T r p P h e G ly Codo ns 3 5 mRN T r a n s l a t i o n 2/3/11 Pepti de bond Fig. 5-18 Amino end (N-terminus) Pepti de bond Side chains Backbo ne Carboxyl end (C-terminus) ( a ) ( b 2/3/11 The ribosome always travels from 5 to 3 on the mRNA. Can you tell whether the N- terminus of the protein is coded by the 5 or 3 end of the mRNA molecule? a. No, it could be either. b. Yes, the 3 end c. Yes, the 5 end 2/3/11 Fig. 17-5 Se cond m RNA base First mRNA base (5 end of codon) Third mRNA base (3 What amino acid is coded by the DNA sequence 5 CCU 3 ? What DNA sequence was used to code the following polypeptide? Met- Ala- His 2/3/11 Figure 6-3 Molecular Biology of the Cell ( Garland Science 2008) 2/3/11 Fig. 17-24 RNA polymerase D N A Polyriboso me mR NA 0.25 m Direction of transcript ion D N A RNA polymer ase Polyriboso me Polypept ide (amino end) Riboso me mRNA (5& end) 2/3/11 Fig. 17-21 Riboso me mR NA Sign al pepti de Signal- recognition particle (SRP) CYTOS OL Translocati on complex SRP recept or protei n ER LUMEN Signal peptid e remov ed ER membra ne Prote in 2/3/11...
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Lecture11_1 - 2/3/11 Main Points Brief review of...

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