Lecture11_1 - Main Points Brief review of translation...

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2/3/11 Main Points Brief review of translation Bacterial gene regulation- operons The many levels of eukaryotic gene regulation
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2/3/11 Fig. 17-13 Polypeptid e Riboso me Ami no acid s tRNA with amino acid attached tRN A Anticod on T r p P h e G ly Codo ns 3 5 mRN A T r a n s l a t i o n
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2/3/11 Pepti de bond Fig. 5-18 Amino end (N-terminus) Pepti de bond Side chains Backbo ne Carboxyl end (C-terminus) ( a ) ( b )
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2/3/11 The ribosome always travels from 5’ to 3’ on the mRNA. Can you tell whether the N- terminus of the protein is coded by the 5’ or 3’ end of the mRNA molecule? a. No, it could be either. b. Yes, the 3’ end c. Yes, the 5’ end
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2/3/11 Fig. 17-5 Second mRNA base First mRNA base (5 end of codon) Third mRNA base (3 codon) What amino acid is coded by the DNA sequence 5 ’CCU 3’ ? What DNA sequence was used to code the following polypeptide? Met- Ala- His
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2/3/11 Figure 6-3 Molecular Biology of the Cell (© Garland Science 2008)
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2/3/11 Fig. 17-24 RNA polymerase D N A Polyriboso me mR NA 0.25 µm Direction of transcript ion D N A RNA polymer ase Polyriboso me Polypept ide (amino end) Riboso me mRNA (5° end)
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2/3/11 Fig. 17-21 Riboso me mR NA Sign al pepti de Signal- recognition particle (SRP) CYTOS OL Translocati on complex SRP recept or protei n ER LUMEN Signal peptid e remov ed ER membra ne Prote in
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2/3/11 Fig. 17-10 mR NA Poly-A tail 5 Ca p 5 UTR 3 UTR 1 1 0 0 What is a gene? In other words, what DNA sequences are needed
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