Lecture11_8 - Main Points Molecular biology techniques DNA...

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2/3/11 Main Points Molecular biology techniques DNA cloning Restriction digests PCR Big picture ideas What is the difference between DNA, reproductive, therapeutic cloning? What are three types of stem cells and
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2/3/11 What is cloning? Cloning is the process of making many identical copies of… …a piece of DNA (DNA cloning) …a type of cell …an organism (reproductive cloning, stem-cells)
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2/3/11 The purpose of DNA cloning To make many copies of a single piece of DNA To produce the protein product of a Fig. 20-2a DNA of chromoso me Cell containing gene of interest Gene inserted into plasmid Plasmid put into bacterial cell Recombinan t DNA (plasmid) Recombina nt bacterium Bacterial chromoso me Bacteri um Gene of intere st Plasm id 2 1 2
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2/3/11 Figure 8-39 Molecular Biology of the Cell (© Garland Science 2008)
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2/3/11 Figure 8-40 Molecular Biology of the Cell (© Garland Science 2008)
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2/3/11 Figure 8-48 Molecular Biology of the Cell (© Garland Science 2008)
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What DNA sequences does a plasmid have? Cloning
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Lecture11_8 - Main Points Molecular biology techniques DNA...

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