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Unformatted text preview: BIOL 429 Paper 3 Discussion Questions RESULTS 1 1. Where is CXCR4 normally localized and why is it localized to the ER in Figure 1B? RESULTS 2 2. Why is lysozyme targeted to the ER in this experiment? 3. Why is there less lysozyme secreted in Figure 2B as compared to Figure 2A? 4. How does the deletion of the C-terminus of the KDEL receptor affect retention of lysozyme? RESULTS 4 5. How does the deletion of the C-terminus affect the movement of the KDEL receptor to the ER? 6. Why is there no movement of the KDEL receptor to the er in the top row of images in Figure 4? RESULTS 5 7. Why is serine replaced by aspartic acid in experiments done in Figure 5? 8. In 5C, what protein is the phosphorylated receptor shown to interact with? RESULTS 6 9. In Figure 6C, what does S209A indicate and how does this replacement affect the behavior of lysozyme-KDEL? RESULTS 7 10. How does Figure 7B support the idea that PKA is phosphorylating serine 209?...
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- Fall '08
- cell biology