BIOL 429 DISC 4 - B I O L 429 P a pe r 4 D is c u s s io n...

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BIOL 429 Paper 4 Discussion Questions Tanabe, K., T. Torii, W. Natsume, S. Braesch-Andersen, T. Watanabe, and M. Satake. 2005. A novel GTPase-activating protein for ARF6 directly interacts with clathrin and regulates clathrin- dependent endocytosis. Molecular Biology of the Cell 16:1617-1628. Arf6 proteins control vesicles with clathrin-coat assemblies. GAP activates the hydrolysis of Arf-bound GTP. These residues form the zinc-finger motif found in various Arf-GAPs. “Mock” indicates that there is no addition of GAP1t or SMAP1t. The membrane protrusions represent where activated Arf6 and F-actin localize together when treated with AlF. Overexpression of SMAP1 does not interfere with the reorganization of actin, because overexpression did not prevent the formation of membrane protrusions. Although the overexpression of full-length GAP1 in the cells disrupted the Golgi structures and did not interfere with the internalization of transferring, overexpression of SMAP1 did not affect the distribution of ß-COP, indicating that SMAP1 is involved in Arf6 pathways but not those regulated by Arf1.
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