BIOL 429 DISC 6

BIOL 429 DISC 6 - BIOL 429 Paper 6 1. What is phos...

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BIOL 429 Paper 6 1. What is phospholipase D and why is it proposed to be involved in receptor-mediated endocytosis? RESULTS 1 2. What is BtOH and how is it used to “disrupt” PLD effects within the cell? 3. Why is PA production used to assay for PLD function in Figure 1? RESULTS 2 4. How does BtOH affect receptor levels in the cell? RESULTS 3 5. What two different aspects of receptor-mediated endocytosis are investigated in Results 3? RESULTS 4 6. Distinguish between RalA WT and RalA S28N. 7. How does overexpression of RalA WT affect removal of the receptor from the plasma membrane? RESULTS 5 8. Why does Go6976 inhibit EGF-induced PLD activity? Phospholipidase D (PLD) is an enzyme that hydrolyzes phosphatidylcholine to form phosphatidic acid and choline. PLD has been implicated in the formation of vesicles, cytoskeletal dynamics and membrane trafficking. The hypothesis that PLD might play a role in receptor-mediated endocytosis accompanied studies of epidermal growth factor (EGF), which is endocytosed upon reception at the cell surface. BtOH is butanol, a four-carbon alcohol, and iso-BtOH is a comparable form of this molecule. PLD shows a
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This note was uploaded on 02/02/2011 for the course BIOL 429 taught by Professor Howe,k during the Fall '08 term at New Mexico.

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BIOL 429 DISC 6 - BIOL 429 Paper 6 1. What is phos...

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