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Unformatted text preview: BIOL 429 PAPER 8 DISCUSSION QUESTIONS SCC = Kleisin (In fig 20-23); Esp1 = Separin RESULTS 1 1. How does Figure 1 show that the phosphorylated form of Scc1 is a better substrate for cleavage by Esp1 cleavage? RESULTS 2 2. How is Scc1 detected in the blot in Figure 2B? 3. How are proteins identified in Figure 2A? RESULTS 5 4. How are the inhibitors used in Figure 5 designed to work? 5. Which is a better inhibitor of cleavage? Explain your answer. RESULTS 6 6. Why doesnt the AMK inhibitor prevent cleavage when used with Esp1 harboring the mutation H1505A? RESULTS 7 7. How do the results shown in Figure 7 indicate that cleavage of Scc1 is enough to trigger anaphase? The researchers treated two samples of chromatin from cells arrested in metaphase. One sample was treated with phosphatase (which would de-phosphorylate Scc1), the other with a control buffer. The phosphatase-treated chromatin was almost completely resistant to cleavage (These all remain in the same region in figure 1C). This suggests that the phosphorylation of Scc1 is highly conducive to cleavage. same region in figure 1C)....
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- Fall '08
- cell biology