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BIOL 429 DISCUSSION 1

BIOL 429 DISCUSSION 1 - BIOL 429 Biology 429 Paper 1...

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BIOL 429 Paper 1 Discussion Questions Biology 429 Paper 1 Discussion Questions Due: Friday, 9/24, in class RESULTS 1 1. In what way has the h14 protein amino acid sequence been altered? 2. Why is pre-prolactin production affected by h14 mutants, but not cyclin production? 3. Why is pPL conversion to PL considered a reflection of translocation efficiency? RESULTS 2 4. What is shRNA used for? 5. What is shRNA (Luc) in contrast to shRNA (14)? 6. Which mutants had the most severe effect on secretion of SEAP? RESULTS 4 7. How is cell growth affected by H14 mutants? CONCLUSIONS 8. How does the A6-12 mutation affect cells as compared to the other two mutations? The h14 protein has been altered in three ways – 14A12 has been altered so that the amino acid sequence from 96 upward is different from the wild type. 14A6-12 has been altered so that the amino acid sequence is identical to 14A12 except that amino acids from 96-100 are identical to wild type. 14A5 has been altered so that amino acids 96-100 are replaced with alanines, and 101 upward are wild type. Amino acids in sequence higher than 107 are not
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