BIOL 429Paper 1 Discussion QuestionsBiology 429Paper 1 Discussion QuestionsDue: Friday, 9/24, in classRESULTS 11. In what way has the h14 protein amino acid sequence been altered?2. Why is pre-prolactin production affected by h14 mutants, but not cyclin production?3. Why is pPL conversion to PL considered a reflection of translocation efficiency?RESULTS 24. What is shRNA used for?5. What is shRNA (Luc) in contrast to shRNA (14)?6. Which mutants had the most severe effect on secretion of SEAP?RESULTS 47. How is cell growth affected by H14 mutants?CONCLUSIONS8. How does the A6-12 mutation affect cells as compared to the other two mutations?The h14 protein has been altered in three ways – 14A12 has been altered so that the amino acid sequence from 96 upward is different from the wild type. 14A6-12 has been altered so that the amino acid sequence is identical to 14A12 except that amino acids from 96-100 are identical to wild type. 14A5 has been altered so that amino acids 96-100 are replaced with alanines, and 101 upward are wild type. Amino acids in sequence higher than 107 are not
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