chap_8-2

chap_8-2 - Prokaryote vs. Eukaryote mRNA Prokaryote...

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Unformatted text preview: Prokaryote vs. Eukaryote mRNA Prokaryote Eukaryote transcription Gene (DNA) Primary transcript (RNA) translation transcription RNA processing Mature mRNA translation polypeptide Brainstorm What are examples of mRNA processing in eukaryotes? 5 Methylated Cap Poly-A-Tail RNA splicing remove introns Alternative splicing 5 Methylated Cap Step 1: Backward G is added by a special capping via a triphosphate bridge Step 2: methyl transferase adds methyls to G and 1+ succeeding nucleotides Fig. 8.13 3 Poly-A-Tail Fig. 8.14 Step 1: ribonuclease cuts mRNA 11-30 nucleotides downstream of AAUAAA (recognition element) Step 2: poly-a-polymerase adds multiple As onto 3 end Step 1 Step 2 Methylated Cap and poly-A tail stabilize mRNA 5 cap Translation initiation factor A A A A Poly-A binding protein RNA splicing removes introns Exons sequences found in a genes DNA and mature mRNA (ex pressed regions) Introns sequences found in DNA but not in mRNA (in tervening regions) Some eukaryotic genes have many introns Fig. 8.16 RNA SPLICING...
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This note was uploaded on 02/03/2011 for the course BIOL 2153 taught by Professor Larkin during the Fall '03 term at LSU.

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chap_8-2 - Prokaryote vs. Eukaryote mRNA Prokaryote...

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