chap_17-2

chap_17-2 - cis-acting elements vs. trans-acting factors...

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Chapter 17, Part II Prokaryotic Gene Regulation
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Genetics and the lactose ( lac ) operon Identification of mutants 1. Lac - mutants lac Z- Beta- galactosidase lac Y- lac permease lac A- not really lac A-, its a trans acetylase that is not essential 2. Constitutive mutants synthesize enzymes. Irrespective of environmental conditions (always “on”)
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lacI - mutants make repressor that can’t bind operator (or don’t make repressor at all) What other lacI mutants might you expect to find? lacls super repressor X Genetics and the lactose ( lac ) operon
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Where would you expect to find each type? ( lacI - & lacI S ) * lacI- can be total loss of repressor or mutation of a specific domain
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RNA
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Genetics and the lactose ( lac ) operon What if I told you there is another type of constitutive mutant? LacOc
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How did they distinguish between lacO c and lacI - mutants? A cis/trans test
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Unformatted text preview: cis-acting elements vs. trans-acting factors lacO c cis lacI -trans Cis/trans test with partial diploids Cis/trans test with partial diploids Cis/trans test with partial diploids Using the lac operon in cloning 1. Identifying transformants with vector + insert 1. As a reporter for gene expression Reporter gene = Gene whose expression is quantifiable by reliable techniques of protein detection Arabidopsis thaliana with a root-specific promoter controlling expression of a reporter gene (B-glucuronidase) Clicker: A mutation affecting the operator-binding site of the repressor would still result in lactose breakdown. 1. True 1. False lactose breakdown Clicker: A mutation affecting the inducer-binding site of the repressor would still result in lactose breakdown. 1. True 1. False lactose breakdown...
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chap_17-2 - cis-acting elements vs. trans-acting factors...

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