BBlecture8S09s - reg19 Regulation of Gene Expression...

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Regulation of Gene Expression Regulation of Gene Expression (sensing, signaling, RNA-based regulation, global controls) (sensing, signaling, RNA-based regulation, global controls) 9.5: Two-component Regulatory Systems 9.5: Two-component Regulatory Systems 9.6: Quorum Sensing 9.6: Quorum Sensing 9.7: Regulation of Chemotaxis 9.7: Regulation of Chemotaxis 9.10: The Stringent Response 9.10: The Stringent Response 9.11: Other Global Control Networks 9.11: Other Global Control Networks 9.16: Attenuation 9.16: Attenuation reg19
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termination of transcription: RNA : single stranded nucleic acid can form secondary structures Rho-dependent termination : protein signal Rho binds to RNA; able to cause RNA & RNA polymerase to leave DNA → termination Rho-independent signal : hairpin or stem-loop RNA structure forms, followed by several uracils → termination reg20
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stem-loop structure in RNA can result in termination of transcription: (Fig 7.23, 12 th ed) reg21
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tryptophan operon tryptophan operon : : tandem tryptophan codons in leader sequence code leader peptide translated when tryptophan is plentiful in cell not synthesized when tryptophan is in short supply when leader peptide is made, transcription of rest of trp operon is terminated (Fig 9.26a, 12 th ed) reg22
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upstream part of trp operon : 1 2 3 4 poly(U) trpE tandem trp codons leader sequence attenuator (after Prescott et al, 2005) region 1: synthesis of leader peptide (trp rich) regions 1, 2: can form stem-loop structure regions 2, 3: can form stem-loop structure regions 3, 4: can form stem-loop structure + polyU rho-independent termination rho-independent termination region 2: can base pair with region 1 or with region 3 reg23
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BBlecture8S09s - reg19 Regulation of Gene Expression...

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