BBlecture19S09s - ster1 Microbial Growth Control...

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Microbial Growth Control Microbial Growth Control (Physical & Chemical Treatments) (Physical & Chemical Treatments) 27.1 27.1 Heat Sterilization Heat Sterilization 27.2 27.2 Radiation Sterilization Radiation Sterilization 27.3 27.3 Filter Sterilization Filter Sterilization 27.4 27.4 Chemical Growth Control Chemical Growth Control 27.5 27.5 Chemical Antimicrobial Agents for Chemical Antimicrobial Agents for External Use External Use ster1
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prevention of microbial growth prevention of microbial growth : : important in : food industry other industrial processes: e.g., biofouling of oil pipelines health care: nosocomial infections drinking water distribution systems strategies include : heat sterilization radiation filtration chemical sterilization ster2
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effect of temperature on viability of a mesophilic bacterium effect of temperature on viability of a mesophilic bacterium : : decimal reduction time (D) : time required for a 10-fold reduction in population density at given temperature thermal death time (TDT) : time required to kill all cells at a given temperature; population size-dependent (Fig 27.1, 12 th ed) ster3
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relationship between temperature and the rate of killing relationship between temperature and the rate of killing : : bacterium “a” = a mesophile bacterium “b” = a thermophile D values (Fig 27.2, 12 th ed) ster4
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an autoclave an autoclave : : steam under pressure enables temperature to reach 121ºC sufficient to destroy endospores as well as vegetative cells in B1-376 in B1-376 (Fig 27.3c, 12 th ed) ster5
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flow of steam through an autoclave flow of steam through an autoclave : : (Fig 27.3a, 12 th ed) ster6
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an autoclave cycle an autoclave cycle : : sterilization exhaust (Fig 27.3c, 12 th ed) ster7
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drying oven : limited usefulness moist heat penetrates better than dry heat ster8
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