Lab Report MB - E nzyme Activity and Efficiency By Maryah...

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Enzyme Activity and Efficiency By: Maryah Barna Abstract Enzymes are proteins that lower the activation energy of reactions, allowing them to occur at a more rapid pace than usual. In these experiments, enzymes are tested to see how pH affects their ability to lower the activation energy. The enzyme used, catecholase should lose its efficiency if the pH is either too high or too low. Not being within catecholase’s optimal pH range will cause the enzyme to denature and reduce its ability to perform. So testing different levels of pH shows which pH is optimal and which starts to denature the enzyme. The results showed that the pH for catecholase is best between 5 and 8, therefore supporting the hypothesis: if the pH of a substance is low, then the enzyme used, catecholase, will lose its efficiency. Thus, in order for catecholase to not denature, the solution it is in must remain between a pH of 5 and a pH of 8. Introduction An enzyme is a protein that lowers the activation energy of a reaction. Enzyme efficiency is affected by the concentrations of the enzyme and the substrate, pH and temperature (Campbell 152). This experiment has two parts to it. The first its to study the effects of pH, or hydrogen concentration, on an enzyme, catecholase, and its ability to lower the activation energy of a reaction. Each enzyme has a certain range of pH that it has optimal effectiveness (Antonio). In the experiment the effects of going outside of this range are studied. The “effects of high pH are analogous to those of low pH” on enzymes, which is denaturation (Ohio). Denaturation is when a protein’s bonds are weakened and begins to uncoil. The hypothesis is that if the pH of a substance is low, then the enzyme used, catecholase, will lose its efficiency. Therefore, the null hypothesis is if the pH of the substance is low
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This note was uploaded on 02/05/2011 for the course BIO 102 taught by Professor Sparace during the Fall '08 term at Tri - County Technical College.

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Lab Report MB - E nzyme Activity and Efficiency By Maryah...

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