{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Study Guide 3 - BCMB3100 Chapter 3 Handout 3 Chapters 3-4...

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Handout 3 BCMB3100 Chapters 3-4 Chapter 3 18) What functional groups or amino acids contribute to the pI of a protein? What is the difficulty in calculating the pI of a protein? 19) What is isoelectric focusing? 20) Know the difference between anion exchange and cation exchange chromatography. Given protein ‘A’ with a pI of 7 and protein ‘B’ with pI of 5. Tell me a strategy (pH and resin) for separating the two using ion exchange chromatography. 21) During Gel Filtration, which elutes first, small proteins or large proteins? 22) How does Affinity Chromatography work, and how would you elute the protein? 23) What is the function of SDS in SDS-PAGE? Which runs faster, small or large proteins. 24) Describe 2-D PAGE Chapter 4 Problems: (1, 3, 7, 11, 12,) 1) Know what the ‘native conformation’ of a protein mean? Know that Alzheimer’s and ‘Mad Cow’ are protein folding diseases. What is a Prion? 2) Know that globular proteins are soluble, compact proteins with a hydrophobic core and a polar/charged surface. Fibrous proteins are structural. Membrane proteins fold differently because they are structured in a
Image of page 1
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern