Study Guide 5 - Handout 6 BCMB3100 Chapter 5 1 What do...

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Handout 6 BCMB3100 Chapter 5 1) What do enzymes do? Do they make unfavorable reactions favorable? Do they affect up-hill reactions? Do they affect K eq ? 2) What limits the rate of an uncatalyzed reaction? What is an enzyme mechanism? 3) Given a non-enzymatic reaction, how would you determine the forward rate constant ( k 1 )? Given k 1 and K eq for a first-order reaction, calculate k -1 . 6) What is meant by ‘pseudo-first order’? What is unique about the order of enzyme reactions? What order is an enzyme reaction at high [S]? What does that mean? 7) What does the rectangular hyperbola mean for a rate curve? What is the shape of the curve if [S] is sat’d and [E] is varied? What is the order of the reaction relative to [E]? 8) In 1890 Emil Fischer introduced the idea of a ‘lock and key’ to describe what? 9) In 1902, Brown and Henri expanded Fischer’s ‘lock and key’ to invoke what kind of complex? What was their evidence? 4) Why do progress curves for enzymes plateau? At least three reasons. 10) Why can we ignore the reverse reaction when we are determining the rate constants for enzymes?
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