MIBO 4220 Worksheet for Article 3

MIBO 4220 Worksheet for Article 3 - 5. In Fig. 3, why was a...

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Work sheet for Article 3: Due before 10 AM on Nov. 19, 2010. Staphylococcus aureus IsdB is a hemoglobin receptor required for heme iron utilization. V. Torres et al. 2006, J. Bacteriol . 188 : 8421. 1. What is the authors’ hypothesis? 2. What experimental data led to this hypothesis? 3. What did the authors tentatively conclude from the data presented in Fig. 2? Why is the conclusion tentative, i.e. what part of the conclusion is clearly demonstrated by the data and what part is implicated, but the “how” is not demonstrated? 4. How did the authors determine the preference of S. aureus for hemoglobin- Fe versus transferrin-Fe? What is likely to be the difference in the mechanism for acquisition of hemoglobin-Fe versus transferrin- Fe?
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Unformatted text preview: 5. In Fig. 3, why was a srtA mutant strain used to help determine what surface protein binds hemoglobin? 6. How did the authors determine that IsdB is the protein that actually binds hemoglobin at the surface? 7. What assays did they use to show that IsdB is also responsible for uptake of heme? 8. How did they show that heme, but not hemoglobin, is imported into the cytoplasm of the bacteria? 9. What role does IsdH play in pathogenesis of S. aureus in the mouse model? What evidence is there from this paper and previous work that suggests a role for IsdH is Fe utilization? 10. Why would IsdB be a good candidate for development of a vaccine against S. aureus infections? How do monooxygenases release iron from heme?...
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MIBO 4220 Worksheet for Article 3 - 5. In Fig. 3, why was a...

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