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Unformatted text preview: 5. In Fig. 3, why was a srtA mutant strain used to help determine what surface protein binds hemoglobin? 6. How did the authors determine that IsdB is the protein that actually binds hemoglobin at the surface? 7. What assays did they use to show that IsdB is also responsible for uptake of heme? 8. How did they show that heme, but not hemoglobin, is imported into the cytoplasm of the bacteria? 9. What role does IsdH play in pathogenesis of S. aureus in the mouse model? What evidence is there from this paper and previous work that suggests a role for IsdH is Fe utilization? 10. Why would IsdB be a good candidate for development of a vaccine against S. aureus infections? How do monooxygenases release iron from heme?...
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- Fall '08