UnknownsReport - Alcaligenes faecalis

UnknownsReport - Alcaligenes faecalis - Growth occurs,...

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UNKNOWNS REPORT FORM Student: Jaime Checkley Unknown #: A 1 Lab TA/Time: Michael MW 4:40 Identification: Alcaligenes faecalis M ORPHOLOGICAL C HARACTERISTICS Cell Shape & Arrangement: Rod shaped & no specific arrangement Colonial Growth on TSAYE: Whole Colony: Irregular Margin : Irregular Elevation: Flat Color: light cream color; translucent Gram’s Reaction: Gram negative (Gm-) Special Stains (optional): N/A Isolation Temperature: 37° P HYSIOLOGICAL C HARACTERISTICS Media 1 : Urea Agar slant Observations Weak Pink Color Interpretation Presence of urease confirmed; organism is able to degrade urea to ammonia and carbon dioxide Incubation Temperature/Time 37° / 48hrs Used as a confirmation test? No Media 2 : Simmons Citrate Agar Observations Media turns blue Interpretation Citrate has been utilized as a carbon source, forming alkaline by-products Incubation Temperature/Time 37° / 48hrs Used as a confirmation test? No Media 3 : Skim Milk Agar Observations
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Unformatted text preview: Growth occurs, media remains cloudy Interpretation Organism does not produce caseinase Incubation Temperature/Time 37 / 48hrs Used as a confirmation test? No Media 4 : Oxidative- Fermentative Observations Both tubes are bluish-green Interpretation Non-saccharolytic; organism cannot utilize the carbohydrate Incubation Temperature/Time 37 / 48hrs Used as a confirmation test? Yes Media 5 : Blood Agar Plate Observations Green pigment tint produced within the blood Interpretation hemolysis Hemolysins present, Partial breakdown of hemoglobin Incubation Temperature/Time 37 / 48hrs Used as a confirmation test? Yes 1 Notes/Explanations (optional): Using my flowchart I was able to determine this unknown to be Alcaligenes faecalis . Using two different conformational tests, I was able to further support my findings and confirm the presence of Alcaligenes faecalis as one of the A 1 unknowns. 2...
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UnknownsReport - Alcaligenes faecalis - Growth occurs,...

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