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UnknownsReport - Staphylococcus aureus

UnknownsReport - Staphylococcus aureus - 37° 48hrs Used as...

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UNKNOWNS REPORT FORM Student: Jaime Checkley Unknown #: A 1 Lab TA/Time: Michael MW 4:40 Identification: Staphylococcus aureus M ORPHOLOGICAL C HARACTERISTICS Cell Shape & Arrangement: staphylococci Colonial Growth on TSAYE: Colonies are large and circular, yellowish colored, with a smooth shiny surface Gram’s Reaction: Gram Positive (Gm+) Special Stains (optional): N/A Isolation Temperature: 37° P HYSIOLOGICAL C HARACTERISTICS Media 1 : Urea Agar slant Observations Pink Color Interpretation Presence of urease confirmed; organism is able to degrade urea to ammonia and carbon dioxide Incubation Temperature/Time 37° / 48hrs Used as a confirmation test? No Media 2 : Baird-Parker Observations Black growth Interpretation Tellurite reduction Incubation Temperature/Time 37° / 48hrs Used as a confirmation test? No Media 3 : Simmons Citrate Agar Observations Media remains green Interpretation Citrate has NOT been utilized as a carbon source Incubation Temperature/Time
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Unformatted text preview: 37° / 48hrs Used as a confirmation test? No Media 4 : Triple Sugar Iron Slant Observations Yellow slant/butt A/A No gas bubble, no black Interpretation Slant reaction: lactose and/or sucrose fermentation to acid Butt reaction: glucose fermentation to acid, no H 2 S Incubation Temperature/Time 37° / 48hrs Used as a confirmation test? Yes Media 5 : Blood Agar Plate Observations Blood pigment is cleared from under and around growth Interpretation β hemolysis Hemolysins present, complete breakdown of hemoglobin Incubation Temperature/Time 37° / 48hrs Used as a confirmation test? Yes 1 Notes/Explanations (optional): Using my flowchart I was able to determine this unknown to be Staphylococcus aureus . Using two different conformational tests, I was able to further support my findings and confirm the presence of Staphylococcus aureus as one of the A 1 unknowns. 2...
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