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Unformatted text preview: 37° / 48hrs Used as a confirmation test? No Media 4 : Triple Sugar Iron Slant Observations Yellow slant/butt A/A No gas bubble, no black Interpretation Slant reaction: lactose and/or sucrose fermentation to acid Butt reaction: glucose fermentation to acid, no H 2 S Incubation Temperature/Time 37° / 48hrs Used as a confirmation test? Yes Media 5 : Blood Agar Plate Observations Blood pigment is cleared from under and around growth Interpretation β hemolysis Hemolysins present, complete breakdown of hemoglobin Incubation Temperature/Time 37° / 48hrs Used as a confirmation test? Yes 1 Notes/Explanations (optional): Using my flowchart I was able to determine this unknown to be Staphylococcus aureus . Using two different conformational tests, I was able to further support my findings and confirm the presence of Staphylococcus aureus as one of the A 1 unknowns. 2...
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- Fall '08
- Glucose, Jaime Checkley Lab, Incubation Temperature/Time, confirmation test, Michael MW