Exercise 16 Extracellular Enzymes

Exercise 16 Extracellular Enzymes - Exercise 16:...

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Exercise 16: Extracellular Enzymes I. Extracellular Enzymes a. Enzyme: proteinaceous substance produced by living organisms that act as biological catalysts to speed up reactions b. Extra(exo)cellular enzymes are excreted by the cell and act to hydrolyze complex molecules (proteins, fats, polysaccharides) that cannot readily enter the cell but whose hydrolyzed products can (amino acids, peptides, fatty acids, etc.) c. Hemolysins: extracellular enzymes produced by many pathogenic bacteria that enhance virulence by destroying the host’s red blood cells d. How to test? If an organism produces an extracellular enzyme, it can usually be detected by visible changes in the test medium; the substrate of the enzyme is incorporated into a solid medium, it is inoculated, and, as the bacteria grows, the enzyme is excreted into the medium and hydrolyzes the substrate, producing a zone of clearing in an otherwise “cloudy” medium e. Protein hydrolysis: Caseinase i. Casein is primary protein in milk and gives it its opaqueness
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This note was uploaded on 02/08/2011 for the course MIBO 3510L taught by Professor Dustman during the Fall '08 term at University of Georgia Athens.

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Exercise 16 Extracellular Enzymes - Exercise 16:...

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