Regulation-1

Regulation-1 - Binding of proteins to DNA increases their...

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Levels of Expression Control Fewer effectors Slower response More effectors Faster response
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Transcription factors contribute to promoter recognition by RNA polymerase (Positive regulators)
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Repressible expression – Negative regulator
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Cis and Trans – Cistrons Operators and Operons
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Negative vs. Positive Control Addition of ligand switches gene off by enabling repressor binding Addition of ligand switches gene off by removing activator protein Addition of ligand switches gene on by removing repressor protein Addition of ligand switches gene on by enabling activator binding
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Combinatorial control of transcription
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Cooperative binding – Positive feedback Sharp transitions between regulated states Important component of regulatory switches e.g. bacteriophage lambda lysogenic/lytic state control
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Action at a distance – enhancers and silencers -
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Unformatted text preview: Binding of proteins to DNA increases their local concentration-Proteins that bend DNA can catalyze interactions Eukaryotic transcription initiation TATA binding protein and other general transcription factors Action at a distance Eukaryotic version Cells containing the same DNA can have very different functions generated through very different gene expression patterns Cellular differentiation generates tissues- organized arrangements of stereotypical cell types Pluripotent stem cells Totipotent stem cells Combinatorial control from a distance Combinatorial determination Positive feedback Persistent response to a transient signal Negative feedback (see question 8-14) Transient response to a persistent signal Chromatin structure plays an important role Chromatin protein patterns must replicate...
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This note was uploaded on 02/09/2011 for the course BIOS 20182 taught by Professor Lahn during the Spring '11 term at UChicago.

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Regulation-1 - Binding of proteins to DNA increases their...

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