Chapter4CellCount

Chapter4CellCount - Lecture 9 Growth Measuring Growth...

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Lecture 9 - Growth Measuring Growth
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Learning Objectives Be able to sketch and label a bacterial growth curve (x- and y-axis as well as phases). Explain what happens in each phase of a bacterial growth curve. Be able to use the growth equation to model bacterial growth. Explain the difference between primary and secondary metabolites, and give an example of each. Explain why it takes a very long time for 100% of the bacteria in a culture to die. Cite three differences between a continuous culture and a batch culture. Know HOW and WHEN to quantitatively measure bacterial growth by each of the following methods Direct counts Petroff-Hauser chamber Coulter counter Viable (Indirect) counts Dilution series + pour plate Dilution series + spread plate Membrane filtration method Most probable number method Biomass measurements Turbidity Total cell mass Know how bacterial growth can be quantified by measuring the accumulation of primary metabolites such as acid and gas, or by measuring ATP formation.
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Vocabulary
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