Chapter4Cultures - Lecture 8 - Growth Cultures and Media...

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Lecture 8 - Growth Cultures and Media
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Learning Objectives Explain how bacterial cell division differs from eukaryotic cell division. Describe a biofilm, and explain the stages of biofilm formation. Explain what it means to say that a bacterial colony is clonal. Understand why it is helpful, but not absolutely necessary, to isolate a bacterial colony before studying the growth and physiology of the cells. Describe three ways to store bacterial cultures long-term. List five categories of bacteria based on optimal growth temperature. Sketch a typical graph of bacterial growth rate vs. temp., and explain the shape of the graph. List five categories of bacteria based on oxygen requirement. Recognize how to distinguish between these 5 types in the lab. Explain the differences in metabolism between the 5 categories listed above. List three categories of bacteria based on optimal pH for growth. Explain two ways in which bacteria prevent plasmolysis in a hyperosmotic environment. List 10 elemental nutrients required for bacterial growth, and explain where bacteria get these nutrients and what cell structures the nutrients are used to build. List the metabolic categories that explain how bacteria obtain carbon, energy and electrons. Be able to categorize a medium as complex or defined based on an ingredients list.
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This note was uploaded on 02/11/2011 for the course BIO 221 taught by Professor Walter during the Spring '10 term at Purdue University-West Lafayette.

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Chapter4Cultures - Lecture 8 - Growth Cultures and Media...

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