Chapter5ControlGrowth

Chapter5ControlGrowth - Lecture 10 Control of Growth...

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Lecture 10 – Control of Growth
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Learning Objectives Explain the differences between sterilization, pasteurization, disinfection, decontamination and sanitization. List three bacterial structures and one eukaryotic structure that are hard to kill. Explain why naked viruses are harder to kill than enveloped viruses. Explain the concept of decimal reduction time, and how it depends on the organism you’re trying to kill, the surface you’re trying to decontaminate, and the decontaminant used. Differentiate between critical, semicritical and non-critical medical instruments. Explain why moist heat is a more effective decontaminant than dry heat. Describe how an autoclave is used. List three methods that have been used to pasteurize liquids. Describe the difference between a membrane filter and a depth (HEPA) filter. Understand what types of radiation can and cannot be used to kill bacteria. List three bacterial structures that can serve as targets for chemical disinfectants. Distinguish between high-level, intermediate-level and low-level disinfectants. Describe the mode of action of alcohol, aldehydes, chlorhexidine, ethylene oxide, halogens, heavy metals, peroxide, phenols and quaternary amines. [NOTE: Flash cards will be the easiest way to study this.] Tell the story of the (in)famous Cryptosporidium outbreak in Milwaukee in 1993 (the largest single water-associated disease outbreak in US history). Explain to a person who is not taking Bio 221 how the overuse of triclosan has led to increased bacterial resistance to this antibiotic.
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Vocabulary Sterilize Pasteurize Disinfectant Antiseptic Decontaminate Sanitize Cyst Naked / Enveloped Virus Decimal reduction time critical / semicritical / non-critical instrument
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This note was uploaded on 02/11/2011 for the course BIO 221 taught by Professor Walter during the Spring '10 term at Purdue.

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Chapter5ControlGrowth - Lecture 10 Control of Growth...

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