Chapter7ReplTranscrip0

Chapter7ReplTranscrip0 - Lecture 14 Genetics DNA...

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Lecture 14 - Genetics DNA Replication and Transcription to mRNA
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Learning Objectives Be able to state the modern biological “central dogma.” Explain what we mean when we say that DNA “carries the instructions to make an organism.” Be able to sketch the basic structure of a DNA double helix. Know where the 5’ and 3’ ends are. Know the base pairing rules. Understand what we mean when we say that DNA polymerase needs a template and a primer. Describe how DNA polymerase can immediately correct an incorrectly formed base pair. Be able to sketch a bacterial chromosome in the process of replication, and point to the replication forks. At each replication fork, be able to identify the leading and lagging strands and the 5’ and 3’ ends. State the function of the following enzymes: DNA polymerase, Helicase, Primase, Gyrase. Using a muted movie, be able to narrate your way through DNA replication. Describe three major differences between DNA polymerase and RNA polymerase. Understand how a bacterial cell knows where to begin transcribing a gene into mRNA. Explain why some promoters are recognized by RNA polymerase better than others.
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This note was uploaded on 02/11/2011 for the course BIO 221 taught by Professor Walter during the Spring '10 term at Purdue.

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Chapter7ReplTranscrip0 - Lecture 14 Genetics DNA...

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