Chapter7Transl0

Chapter7Transl0 - Lecture 15 - Genetics mRNA translation to...

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Lecture 15 - Genetics mRNA translation to protein
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Learning Objectives Describe the process of mRNA splicing in eukaryotes. Given the structure of a ribosome, and the 5’ end of a translated mRNA, identify the A, P and E sites. Understand the principle of the genetic code – having three-base codons represent amino acids. Know the codon that initiates protein synthesis (5’AUG 3’). Understand why the ribosome must determine the correct reading frame for the mRNA. Explain how ribosomes know where to start reading the mRNA in both prokaryotes and eukaryotes. Understand how tRNAs ensure that a mRNA codon is represented by the correct amino acid. Understand how aminoacyl-tRNA synthetases ensure that a tRNA is charged with the correct amino acid. Be able to sketch a charged tRNA attached to a mRNA. Label all 5’ and 3’ ends. Given a muted animation, be able to narrate your way through the process of protein synthesis. Explain why occasional “readthrough” of a stop codon does not damage a cell. Be able to sketch the coupled processes of transcription and translation, labeling all 5’ and 3’ ends,
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This note was uploaded on 02/11/2011 for the course BIO 221 taught by Professor Walter during the Spring '10 term at Purdue.

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Chapter7Transl0 - Lecture 15 - Genetics mRNA translation to...

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