Handout - Biotechnology

Handout - Biotechnology - Biotechnology 1. What is...

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Biotechnology 1. What is biotechnology? the use of living organisms to create products or to facilitate processes 2. 97% of human DNA is ( non-coding ). This includes promoter and enhancer sequences, introns, and repetitive DNA. 3. What is the genetic code? Is it the same in all species? It is the sequence of bases that correspond to the different amino acids and it is universal in all organisms 4. T or F: One restriction enzyme is specific for one sequence of DNA bases. 5. How does a restriction enzyme work? One restriction enzyme is specific for one sequence of DNA bases. The restriction enzyme recognizes that series of bases as the place for it to cut. Once the DNA is cut, “sticky ends” are created because the restriction enzyme leaves a stretch of unpaired bases at each end of the cut. These “sticky ends” are open to base pair with a different sequence of DNA that is to be inserted. Boxed area = recognized sequence Red bases = sticky ends Blue bases = new, inserted DNA sequence AGCCATGCCATT AGCCA TGCC ATT AGCCATGCG AATC TGCC ATT TCGGTACGGTAA TCGGT ACGG TAA TCGGT ACGG TTAG ACGGTAA 6. What is the name given to the DNA that results from using a restriction enzyme (DNA that has been combined into a sequence that does not exist already in nature)? Recombinant DNA 7. What is a plasmid? an extra bit of DNA that replicates separately from chromosomes 8. What was the purpose of using plasmids in the insulin example Dr. W gave in class? To have bacterial cells make human insulin (so that diabetics did not have to use the
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This note was uploaded on 02/08/2011 for the course BIO 101 taught by Professor Cantwell during the Fall '08 term at University of Tennessee.

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Handout - Biotechnology - Biotechnology 1. What is...

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