E3 lecture4 - biotechnology

E3 lecture4 - biotechnology - BIOTECHNOLOGY I Human Genome...

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BIOTECHNOLOGY I Human Genome Project - sequencing of all 3 billion base pairs (25,000 genes making 100,000 proteins) 97% of human DNA is non-coding - includes promoter and enhancer sequences - includes introns - includes repetitive DNA Knowing the sequence of DNA is useful to elucidation of evolutionary relationships Biotechnology: Use of living organisms to create products or to facilitate processes Manipulation and movement of DNA between species: Genetic code is universal… genetic code shared by bacteria; humans Concept One: chopping up DNA - Restriction enzyme - A type of enzyme that recognizes a specific series of DNA bases and cuts the DNA strand at that site. Recognition sequences are recognized by the restriction enzymes (leaves “sticky ends”) Recombinant DNA: two or more segments of DNA that have been combined into a sequence that does not exist in nature Concept Two: Plasmids - small ring of DNA outside bacterial chromosome - replicates independent of chromosome - can become incorporated into bacterial chromosome STEPS (insulin example) - Gene for human insulin is cut out using restriction enzyme - Same enzyme cuts plasmid, leaving complimentary ends
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This note was uploaded on 02/08/2011 for the course BIO 101 taught by Professor Cantwell during the Fall '08 term at University of Tennessee.

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E3 lecture4 - biotechnology - BIOTECHNOLOGY I Human Genome...

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