226-exam3 - MIC 226 EXAM III FALL 95 PRINT NAME READ THE...

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MIC 226 EXAM III FALL 95 PRINT NAME________________________________ READ THE QUESTIONS CAREFULLY 1. ( 10 pts) Name five enzymes or other proteins you would expect a phage or animal virus chromosome to encode. This question does not mean that the protein must be encoded by both phages and animal viruses. Either/or is accceptable. A. _____________________________________________________________________________ B. _____________________________________________________________________________ C. _____________________________________________________________________________ D. _____________________________________________________________________________ E. _____________________________________________________________________________ 2. ( 16 pts) List and briefly describe any four of the major steps or biochemical events required for prophage formation. What enzymes, other proteins, nucleic acids, or specific sites within nucleic acids are involved in each step? You may start with lambda, which is a temperate phage, and susceptible bacterial host cells. A. ___________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ B. ___________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ C. ___________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________ D. ___________________________________________________________________________ _______________________________________________________________________________________ _______________________________________________________________________________________
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PRINT NAME_____________________________ 3. (22 pts) Compare and contrast bacterial transformation by chromosomal fragments and conjugation which involves transfer of chromosomal DNA fragments (that is, conjugation involving HFR donors; HFR donors are those with the Fertility Factor integrated in the chromosome). Use complete sentences. In both A and B below, transformation and conjugation refer to these two cases specifically and not to other examples of transformation or conjugation.
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