326R BACTERIOPHAGES - 1 BACTERIOPHAGES BACTERIOPHAGES...

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BACTERIOPHAGES BACTERIOPHAGES 1
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BACTERIOPHAGES - LYTIC GROWTH AND LYSOGENY PHAGE STRUCTURE Capsid, Capsomer, chromosome Phage T4 (Head, Collar, Tail, Core, Sheath, Base plate, Spikes, Tail fibers, Specificity, Double -stranded linear chromosome) LYTIC PHAGE GROWTH Attachment (adsorption, specificity) Penetration (injection) Replication - Transcription, translation - Host provides: energy, ribosomes, RNA polymerase. low molecular weight precursors for macromolecular synthesis - Production of viral proteins and nucleic acids Assembly (maturation) (packaging) intact progeny viruses produced Lysis - release of progeny Burst size Plaques (Host, Lawn, Plaques) Phage growth in liquid cultures of host Phages are said to “infect” their host Phage preparations (i.e., suspensions of phages in liquid) are also called “phage lysates” TEMPERATE PHAGES AND LYSOGENY Lambda - Infection : Attachment, Penetration, Circularization of chromosome. Repression of lytic genes, Integration, Attachment site, Lysogeny, Lysogenic immunity, Prophage, Lysogen Prophage Induction Inducing agent Repression abolished, Lytic gene expression. Excision Lytic growth How would you isolate a lysogenic culture if you are given a temperate phage preparation and a culture of host bacteria? 2
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3 PHAGE STRUCTURE CAPSOMERS- STRUCTURAL PROTEIN ICOSAHEDRON CHROMOSOME: SINGLE-STRAND CIRCULAR DNA 5,386 NUCLEOTIDES 10 GENES 30 nM TWENTY TRIANGULAR PLATES NUCLEO - CAPSID CAPSID φ X174 TMV - TOBACCO MOSAIC VIRUS CHROMOSOME: SINGLE-STRAND LINEAR RNA ~6,000 NUCLEOTIDES CAPSOMERS INFECTIOUS ONLY RNA AND PROTEIN CRYSTALS
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4 PHAGE T4 HEAD TAIL CAPSOMER CORE SHEATH COLLAR BASE PLATE TAIL FIBER (6)
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326R BACTERIOPHAGES - 1 BACTERIOPHAGES BACTERIOPHAGES...

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