BACTERIOPHAGES - LYTIC GROWTH AND LYSOGENY
Capsid, Capsomer, chromosome
Phage T4 (Head, Collar, Tail, Core, Sheath, Base plate, Spikes, Tail fibers, Specificity,
Double -stranded linear chromosome)
LYTIC PHAGE GROWTH
Attachment (adsorption, specificity)
Replication - Transcription, translation
- Host provides: energy, ribosomes, RNA polymerase.
weight precursors for macromolecular synthesis
- Production of viral proteins and nucleic acids
Assembly (maturation) (packaging) intact progeny viruses produced
Lysis - release of progeny
Plaques (Host, Lawn, Plaques)
Phage growth in liquid cultures of host
Phages are said to “infect” their host
Phage preparations (i.e., suspensions of phages in liquid) are also called “phage lysates”
TEMPERATE PHAGES AND LYSOGENY
Lambda - Infection : Attachment, Penetration, Circularization of chromosome.
Repression of lytic genes, Integration, Attachment site, Lysogeny,
Lysogenic immunity, Prophage, Lysogen
Inducing agent Repression abolished, Lytic gene expression.
How would you isolate a lysogenic culture if you are given a temperate phage
preparation and a culture of host bacteria?