050328PlasmidsMobileDNA - 1 MICROBIAL GENETICS MICROBIAL...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 1 MICROBIAL GENETICS MICROBIAL GENETICS PLASMIDS PLASMIDS OTHER MOBILE GENETIC OTHER MOBILE GENETIC ELEMENTS ELEMENTS 2 MICROBIAL GENETICS - PLASMIDS / OTHER MOBILE GENETIC ELEMENTS PLASMIDS Characteristics, Resistance Factors, Resistance Transfer Factors Drug Resistance Mechanisms Penicillin Resistance, Penicillinase, Beta-lactamase Multiple Drug Resistance Transformation by Plasmids OTHER MOBILE GENETIC ELEMENTS Insertion Sequences Transposons Integrons Superintegrons Conjugative Transposons Genomic Islands The Busy Genome 3 PLASMIDS: SMALL, CIRCULAR, DOUBLE-STRAND DNA MOLECULES ~ 5 - 50 GENES, CYTOPLASMIC LOCATION, NOT ESSENTIAL (NORMALLY) REPLICATION GENES AND SITES 1 - 20 COPIES EACH / CELL SEVERAL DIFFERENT PLASMIDS / CELL RESISTANCE FACTORS - PLASMIDS WHICH CARRY GENES WHICH ENCODE PROTEINS WHICH MAKE THE BACTERIAL HOST RESISTANT TO ANTIBIOTIC - CALLED DRUG RESISTANCE RESISTANCE TRANSFER FACTORS - ALL ABOVE PLUS ABILITY TO TRANSFER PLASMID IN MATING [CONJUGATION] OTHER PLASMID GENES: HYDROCARBON CATABOLISM TOXIN PRODUCTION MINERAL UPTAKE 4 MECHANISMS OF DRUG RESISTANCE 1. MUTATION RESULTS IN ALTERED BACTERIAL PROTEIN. IT NO LONGER RECOGNIZES ANTIBIOTIC BUT CONTINUES TO PERFORM NORMAL FUNCTION IN BACTERIAL GROWTH EX: STREPTOMYCIN 2. BACTERIA ACQUIRE NEW GENE WHICH CODES FOR ENZYME WHICH DESTROYS ANTIBIOTIC EX: PENICILLINASE DESTROYS PENICILLIN 3. BACTERIA ACQUIRE NEW GENE WHICH CODES FOR ENZYME WHICH PUMPS ANTIBIOTIC BACK OUTSIDE CELL EX: RESISTANCE 5...
View Full Document

Page1 / 21

050328PlasmidsMobileDNA - 1 MICROBIAL GENETICS MICROBIAL...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online