050328Transduction - 1 MICROBIAL GENETICS MICROBIAL...

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Unformatted text preview: 1 MICROBIAL GENETICS MICROBIAL GENETICS TRANSDUCTION TRANSDUCTION 2 MICROBIAL GENETICS - TRANSDUCTION GENERALIZED TRANSDUCTION Two step procedure Growth of phage on donor host producing some transducing particles in the phage prep Infection of recipient host by transducing particles Phage P1, Growth of phage on donor host Transducing Particles, Transducting phage preps (lysates) Donor Host, Recipient Host Infection of recipient culture by transducing phage prep Recombination - Homologous, Breaking and Rejoining Selection Transductants, Recombinants SPECIALIZED TRANSDUCTION Three step procedure Lysogenization of donor host Induction of lysogenic culture producing transducing particles Infection of recipient host by transducing particles Phage Lambda Infection of Donor Host Lysis or Lysogeny Prophage Attachment Site, Prophage, Lysogen Induction of lysogen, Lytic growth Illegitimate recombination, transducing particles, transducing phage prep Infection of recipient culture, Integration Selection Transductants Partial diploid transductants 3...
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This note was uploaded on 02/08/2011 for the course BIO 326R taught by Professor Whiteley during the Spring '08 term at University of Texas at Austin.

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050328Transduction - 1 MICROBIAL GENETICS MICROBIAL...

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