050330Conjugation - 1 MICROBIAL GENETICS CONJUGATION 2...

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1 MICROBIAL GENETICS MICROBIAL GENETICS CONJUGATION CONJUGATION
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2 MICROBIAL GENETICS - CONJUGATION Male, Fertility Factor, Donor Female, absence of F Factor, Recipient F+ Male, Sex Pili, Conjugation Bridge, Origin of Transfer, Nicking at oriT, Transfer of Single Strand, Complementary strand synthesis in male and female Hfr Male (High Frequency of Recombination), Integration, Homologous Recombination, Selection of recombinants F' Male, Illegitimate recombination, Partial diploids
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3 CONJUGATION - MATING OF MALE AND FEMALES E. COLI MALES: FERTILITY FACTOR F PLASMID ~100,000 BASE PAIRS MALE CHARACTERISTICS CONTACT WITH FEMALE DNA DONATION FEMALES: F - DNA RECIPIENT ABSENCE OF F FACTOR F FACTOR - DOUBLE STRAND DNA IS2 INSERTION SEQUENCE 1 REPLICATION GENES oriT ORIGIN OF TRANSFER TRANSFER GENES F
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4 F + MALE BINARY FISSION F PILUS CHROMOSOME AND F FACTOR DUPLICATED
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5 F FACTOR TRANSFER: F + x F - F + AND F + F - F + NICK F AT oriT TRANSFER ONE STRAND CONJUGATION BRIDGE NEW SYNTHESIS= F + F +
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050330Conjugation - 1 MICROBIAL GENETICS CONJUGATION 2...

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